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In the current study, results of both the fecal and clinical samples supported our original hypothesis that H. pancybiotica populations are most likely to be present in the gastrointestinal tract of rats exposed to the antimicrobial agents or antibiotics prosobranched to them. These were the main reasons for increased antibiotic sensitivity observed in the treated groups. As reported in previous studies, there was no increased sensitivity in untreated mice, and the number of animals with MHC class II–restricted resistance was high in both treated groups. This results in a substantial proportion of mice as MHC II–restricted that may not have developed true drug resistance. The findings may point to some potential sources of antibiotic resistance in the developing world, where prevalence of antibiotic use is relatively low. It could be that antibiotics are administered to young animals with decreased susceptibility erythromycin creme bestellen that would predispose them to Buy metformin online australia resistance during long-term use. Such a mechanism could be partly responsible for the increased antibiotic sensitivity present in this study.
The results showed increased antibiotic sensitivity in the first week after treatment started, which coincided with a significant increase in the number of MHC–restricted bacteria (Fig. 1).
Figure 1. View largeDownload slide Increased antibiotic sensitivity of the fecal microbiota with antimicrobial agents among treated adult male GFP-L mice compared with wild-type littermates. The fecal microbiota of untreated and antibiotic-treated groups were analyzed by gel electrophoresis (A) and 16S rRNA gene sequence analysis (B). Littermates were designated as antibiotic-treated if, at a cutoff of 0.5 colony forming unit for the sample, number of bacteria from 10 its 12 animals was less than the number of bacteria from 5 11 animals of the control (untreated) group. Averages were taken over the course of 6 weeks, but this value was not significantly different than the control group during entire time period. (C) Summary of the 16S rRNA gene amplification and PCR primers used to produce the MHC-I and MHC-II specific amplicons in each analyzed group. The primer pairs are presented in bold type.
Figure 1. View largeDownload slide Increased antibiotic sensitivity of the fecal microbiota with antimicrobial agents among treated adult erythromycin salbe bestellen male GFP-L mice compared with wild-type littermates. The fecal microbiota of untreated and antibiotic-treated groups were analyzed by gel electrophoresis (A) and 16S rRNA gene sequence analysis (B). Littermates were designated as antibiotic-treated if, at a cutoff of 0.5 colony forming unit for the sample, number of bacteria from 10 its 12 animals was less than the number of bacteria from 5 11 animals of the control (untreated) group. Averages were taken over the course of 6 weeks, but this value was not significantly different than the control group during entire time period. (C) Summary of the 16S rRNA gene amplification and buy erythromycin tablets 500mg PCR primers used to produce the MHC-I and MHC-II specific amplicons in each analyzed group. The primer pairs are presented in bold type.
The low rate of MHC II-restricted bacteria reported.
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